Aiming at providing an effective method for rapid detection of Foot-and-Mouth
Disease Virus (FMDV), this study developed an antigen-capture reverse transcriptase
loop-mediated isothermal amplification method (Ag-RT/LAMP). Diluted FMDV were
bond by the tubes coated with IgG of FMDV and then detected by Loop-mediated
isothermal amplification (LAMP). This method could detect as few as 0.58x102
copies of virus and showed higher sensitivity than RT-PCR and could also differentiate
FMDV serotypes with high sensitivity and specificity, but if the samples contain
high concentrations of virus this serotype specificity may be unstable. In addition,
the entire reaction required only a single incubation at 63°C for 3 h. The
amplification products could be visually inspected for color change. Ag-RT/LAMP
is sensitive, serotype specific, cost-effective, time-saving, and versatile,
with the potential for analysis of field clinical specimens in developing countries
for FMDV surveillance.
Hongxing Guan, Zhiyong Li, Xiangping Yin, Yun Zhang, Peng Gao, Yinmei Bai and Jixing Liu, 2013. Rapid Detection and Differentiation of Foot and Mouth Disease Virus Serotypes by Antigen-capture Reverse Transcriptase Loop-mediated Isothermal Amplification. Asian Journal of Animal and Veterinary Advances, 8: 647-654.