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Asian Journal of Animal and Veterinary Advances
  Year: 2013 | Volume: 8 | Issue: 4 | Page No.: 647-654
DOI: 10.3923/ajava.2013.647.654
Rapid Detection and Differentiation of Foot and Mouth Disease Virus Serotypes by Antigen-capture Reverse Transcriptase Loop-mediated Isothermal Amplification
Hongxing Guan, Zhiyong Li, Xiangping Yin, Yun Zhang, Peng Gao, Yinmei Bai and Jixing Liu

Abstract:
Aiming at providing an effective method for rapid detection of Foot-and-Mouth Disease Virus (FMDV), this study developed an antigen-capture reverse transcriptase loop-mediated isothermal amplification method (Ag-RT/LAMP). Diluted FMDV were bond by the tubes coated with IgG of FMDV and then detected by Loop-mediated isothermal amplification (LAMP). This method could detect as few as 0.58x102 copies of virus and showed higher sensitivity than RT-PCR and could also differentiate FMDV serotypes with high sensitivity and specificity, but if the samples contain high concentrations of virus this serotype specificity may be unstable. In addition, the entire reaction required only a single incubation at 63°C for 3 h. The amplification products could be visually inspected for color change. Ag-RT/LAMP is sensitive, serotype specific, cost-effective, time-saving, and versatile, with the potential for analysis of field clinical specimens in developing countries for FMDV surveillance.
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How to cite this article:

Hongxing Guan, Zhiyong Li, Xiangping Yin, Yun Zhang, Peng Gao, Yinmei Bai and Jixing Liu, 2013. Rapid Detection and Differentiation of Foot and Mouth Disease Virus Serotypes by Antigen-capture Reverse Transcriptase Loop-mediated Isothermal Amplification. Asian Journal of Animal and Veterinary Advances, 8: 647-654.

DOI: 10.3923/ajava.2013.647.654

URL: http://scialert.net/abstract/?doi=ajava.2013.647.654

 
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