The purpose of this study was to evaluate the role of Interleukin-1β (IL-1β) in Insulin Resistance (IR) during normal pregnancy. This cross sectional study was carried out on 86 healthy pregnant women including 26, 23 and 37 individuals in the 1st, 2nd and 3rd trimesters, respectively and in 21 healthy non pregnant women. Serum IL-1β and resistin concentrations were measured by Enzyme Linked Immunosorbent Assay (ELISA) method. Insulin resistance value was calculated using the homeostasis model assessment, (Homeostasis Model Assessment) HOMA-IR. Serum resistin concentration was found to be significantly raised in the second and third trimester as compared with women with first trimester of pregnancy but it was not found any statistical difference in serum resistin concentration between the healthy controls and pregnant women. There was no difference in IL-1β level between pregnant 1.32±0.48 ng mL-1 and non pregnant women 1.44±0.49 ng mL-1. IL-1β level were significantly decreased with increase in gestational age. Pregnant women exhibited higher score of HOMA-IR 2.1±0.9 compared non pregnant women 1.7±0.4 but there were not difference in this score between pregnant subjects in different gestational age. There was not any correlation between maternal gestational age and serum level of IL-1β. The findings of this study suggest that IL-1β do not appear to contribute greatly to pregnancy induced insulin resistance in healthy pregnancy.