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Asian Journal of Epidemiology
  Year: 2017 | Volume: 10 | Issue: 2 | Page No.: 89-100
DOI: 10.3923/aje.2017.89.100
Livestock-Associated Staphylococcus aureus Pathogenicity with Regards to Resistance and Virulence Genomics and Accessory Gene Regulator Locus Proteomics
Ashraf Samir Hakim , Nesreen Allam Tantawy Allam , Sohier Mohamed Syame , Doaa Sedky, Riham Hassan Hedia and Mohamed Abdel Zaher Elshafaie

Abstract:
Background and Objective: Livestock-associated multidrug-resistant Staphylococcus aureus (LA-MRSA) pathovars with characteristic genetic profiles could lead to differences in antimicrobial responsiveness, reduced treatment susceptibility, however, increased pathogenicity. In this study the prevalence of some LA-MRSA pathovars with characteristics genomic and metabolomics profiles within 3 animal species in specific epidemiological settings in Egypt were investigated. Materials and Methods: The study included small-holders farmers from 10 localities samples collected during 2014- 2016. The focus was exclusively on individuals with untreated skin abscess (pigs), recurrent mastitis (cows), apparently healthy carriers (goats). The interrelationships were evaluated among novel isolates with regards to agr locus expression, antimicrobial resistance pattern, virulence factors genetic profiles and antimicrobial-induced time to clearance of MRSA bacteremia. A chi-square test had estimated the prevalence of each gene profile where differences are significant at p<0.05 (SPSS 19). Results: Six hundred MRSA field isolates molecularly classified by 16S rDNA amplification (241 bp), sequencing, then blastn to genbank data base were included. The virulence factors; clumping factors A protein (clfA = 638 bp), thermo-nuclease (nuc = 395 bp), toxic shock syndrome toxin (tsst = 326 bp) and exfoliative toxins (etb = 226 bp) genes, were determined. Moreover, the accessory gene regulator locus (agr) expression in relation to α and δ Hemolysis grad was estimated that ranged from 0- +4 in a strain’s virulence-dependent manner. In addition, antimicrobial resistance genes profiles were determined to guide treatment trails; methicillin (mecA = 310 bp), vancomycin (vanA =1030 bp) and erythromycin (ermC = 295 bp). Finally, the antimicrobial-induced time to clearance of LA-MRSA bacteremia were compared between studied animal species. Conclusion: It was concluded that clearance of LA-MRSA was significantly dependent on the interactions of the virulence factors, antimicrobial resistance genes and the agr locus expression in relation to hemolysis grad. Hence, considering these future strains of unknown pathogenicity, they could reveal sub-acute, untreated, recurrent and/or food poising diseases.
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How to cite this article:

Ashraf Samir Hakim, Nesreen Allam Tantawy Allam, Sohier Mohamed Syame, Doaa Sedky, Riham Hassan Hedia and Mohamed Abdel Zaher Elshafaie, 2017. Livestock-Associated Staphylococcus aureus Pathogenicity with Regards to Resistance and Virulence Genomics and Accessory Gene Regulator Locus Proteomics. Asian Journal of Epidemiology, 10: 89-100.

DOI: 10.3923/aje.2017.89.100

URL: http://scialert.net/abstract/?doi=aje.2017.89.100

 
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