Abstract:
The purpose of this study was to characterization of
xylanase producing Fusarium solani isolate and optimization of
cultural conditions for xylanase enzyme production. Screening of Fusarium
solani isolate was based on the diameter of the clear zone formation
in oat spelt xylan agar plates, Fusarium solani isolate F7 was
selected and optimized for xylanase enzyme production using cheaper substrate
like wheat straw, rice straw, rice bran and wood husk. Maximum enzyme
activity was observed in wheat straw (78.32 U mL-1). Optimum
pH and temperature for xylanase activity were found to be 5.5 and 30°
at 3% substrate concentration. In purification step, 75% ammonium sulphate
saturation was found to be suitable giving maximum xylanase activity.
Purified xylanase yielded single band with a molecular weight of 89 kDa.
The use of wheat straw as a major carbon source is particularly valuable
because oat spelt xylan is very expensive, The Fusarium solani
F7 isolate proved to be a promising microorganism for xylanase production.
V.K. Gupta, R. Gaur, N. Gautam, P. Kumar, I.J. Yadav and N.S. Darmwal, 2009. Optimization of Xylanase Production from Fusarium solani F7. American Journal of Food Technology, 4: 20-29.
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