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American Journal of Food Technology
  Year: 2009 | Volume: 4 | Issue: 2 | Page No.: 79-89
DOI: 10.3923/ajft.2009.79.89
Effects of Preheated Treatments on Physicochemical Properties of Resistant Starch Type III from Pullulanase Hydrolysis of High Amylose Rice Starch
J. Pongjanta, A. Utaipattanaceep, O. Naivikul and K. Piyachomkwan

Abstract:
In this study, the effects of preheated treatments on physicochemical properties of resistant starch type III formation by pullulanase hydrolysis of High Amylose Rice Starch (HARS) were investigated. A debranching enzyme (Pullulanase, 8 U g-1 starch at 55°C for 0-48 h) was introduced to modify the amylopectin molecules of 15% (w/w) HARS suspension (32.10%, amylose content) which had been preheated at 95 and 121°C for 30 min. Retrogradation gels of debranched starches with different degrees of hydrolysis (0.14 to 3.10%) were then induced at 4°C for 16 h. Afterward, one cycle of the freeze-thaw process (-10/30°C) was applied to promote syneresis of the retrograded starches. Results show that pullulanase hydrolysis enhanced the degree of syneresis (33.22, 45.27 and 58.91% for non-debranched and debranched starches which had been preheated at 95 and 121°C for 48 h, respectively). The debranched starches with higher degree of hydrolysis provided products with higher resistant starch contents. The resistant starch content increased quadrupled with debranching and the freeze-thaw process (4.07 to 10.68% and 5.12 to 19.32% for 0 to 48 h pullulanase hydrolysis of HARS preheated at 95 and 121°C, respectively). Results had shown that after debranching and retrogradation, the HARS molecules had rearranged and changed their crystal pattern from A to V-type pattern, as revealed by X-ray diffraction analysis. In vitro starch hydrolysis index of the RS III samples from 0 to 48 h of pullulanase hydrolysis of the HARS which had been preheated at 95 and 121°C were reduced from 71.591 to 41.69% and 68.66 to 26.83%, respectively.
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How to cite this article:

J. Pongjanta, A. Utaipattanaceep, O. Naivikul and K. Piyachomkwan, 2009. Effects of Preheated Treatments on Physicochemical Properties of Resistant Starch Type III from Pullulanase Hydrolysis of High Amylose Rice Starch. American Journal of Food Technology, 4: 79-89.

DOI: 10.3923/ajft.2009.79.89

URL: http://scialert.net/abstract/?doi=ajft.2009.79.89

COMMENTS
11 November, 2009
muhammad susilo:
This document has helped the writing of my research report. I've researched about Resistant starch Type III from Taro (Colocassia esculenta L.Schot). Anybody can help me more to give me some articles please send to me. My E-MAIL address: muhammadsusilo@yahoo.com Thanks.
 
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