Abstract:
Full length cDNA of wheat MCM2 was used to synthesize the complete MCM2 protein in E. coli. The polyclonal antibodies against the synthesized protein was generated in the rabbit and used for cellular localization of the MCM2 protein during cell cycle. The immunostaining of root cells of wheat shows that MCM2 was localized inside the nucleus and exhibits cyclic synthesis and degradation during cell cycle. On the other side, the over expression of wheat MCM2 protein in Saccharomyces cerevisiae was associated with increase in cell size and protrusion of several buds at different points on the cell surface.
Subscribe Today 
