The morphogenetic potential of leaf callus cultures of Plumbago zeylanica was investigated to develop reliable protocols for shoot regeneration and somaclonal variation. Maximum callus proliferation was obtained Murashige and Skoog (MS) medium supplemented with 2.0 mg L-1 BAP. The maximum shoot regeneration (16.3±0.51) was achieved in five weeks when callus cultured on MS medium containing 0.75 mg L-1 BAP, 1.0 mg L-1 IAA and NAA each. Regenerated shoots were rooted on half strength MS medium supplemented with 0.5 mg L-1 NAA. The rooted plantlets were successfully established in soil. Calli derived from leaf explants cultured on MS medium fortified with 2.0 mg L-1 BAP, when subcultured on MS medium fortified with 2.0 mg L-1 BAP, 1.5 mg L-1 Kin and 1.0 mg L-1 NAA induced somaclonal variation.