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Asian Journal of Plant Sciences
  Year: 2015 | Volume: 14 | Issue: 2 | Page No.: 66-71
DOI: 10.3923/ajps.2015.66.71
Utilization of Sago Palm (Metroxylon sagu Rottb.) Zygotic Embryos for Cryopreservation
A.G.D. Delima, O.P. Damasco and A.U. Novero

Abstract:
The value of sago palm (Metroxylon sagu Rottb.) as a high starch yielding crop has not been fully realized yet but at risk to human-mediated and environmental threats that necessitate its conservation. Attempts were made to investigate cryopreservation as an option for long-term storage of sago palm zygotic embryos using simple desiccation technique. Zygotic embryos were aseptically removed from white sago palm seeds. Laminar desiccation (1-3 h) and silica gel drying (1-7 h for 50, 100 and 150 g) were employed to reduce moisture content without losing much viability. Viability was assessed through tetrazolium test and germination in modified Murashige and Skoog media. Desiccation of embryos in 100 g silica gel for 6 h was the selected pre-treatment to cryopreservation. The critical moisture content of embryos was at 21 with 55% viability. With storage in liquid N, staining showed 14-46.7% partial viability after 2 and 24 h, respectively. High desiccation sensitivity of immature sago embryos hindered its direct storage to liquid nitrogen which suggests complementing simple dehydration techniques with cryoprotectants. Cryopreservation of sago palm remains a potential and progressing approach for future long-term conservation and management of this versatile starch crop.
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How to cite this article:

A.G.D. Delima, O.P. Damasco and A.U. Novero, 2015. Utilization of Sago Palm (Metroxylon sagu Rottb.) Zygotic Embryos for Cryopreservation. Asian Journal of Plant Sciences, 14: 66-71.

DOI: 10.3923/ajps.2015.66.71

URL: http://scialert.net/abstract/?doi=ajps.2015.66.71

 
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