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Biotechnology

Year: 2005 | Volume: 4 | Issue: 4 | Page No.: 288-295
Research Article

A Sucrose Synthase Gene from Broccoli: cDNA Cloning, Sequencing and its Expression During Storage

Bimal Kumar Pramanik
Faculty of Agriculture, Kagawa University, Kagawa, Japan
Toshiyuki Matsui
Faculty of Agriculture, Kagawa University, Kagawa, Japan
Haruo Suzuki
Faculty of Agriculture, Kagawa University, Kagawa, Japan
Yusuke kosugi
Faculty of Agriculture, Kagawa University, Kagawa, Japan
The rapid senescence of broccoli head after harvest at ambient temperatures is accompanied by rapid drop of sucrose from florets. To understand such biochemical changes, we isolated a cDNA for Sucrose Synthase (SS) from broccoli branchlets. The cDNA clone, BoSS (B. oleracea sucrose synthase) (AB212623) encodes an mRNA of 608 bp. The deduced amino acid sequence is highly homologous to SS from Arabidopsis, cotton, mungbean and pea. Transcripts of BoSS were present in both branchlets and florets throughout the storage period. The SS enzyme activity of both portions gradually increased throughout the storage duration except for a transient decrease on the 48 h of storage. However, transcripts were only consistent with enzyme activity up to 24 h of storage. The initial increase in enzyme activity and transcripts level after 24 h of storage caused about 50% decline of sucrose content.
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