Abstract:
Nodal segments, 1.5-3.0 cm long with 1 or 2 nodes taken from in vitro shoot cultures of six jojoba strains, were cultured on MS medium supplemented with 2.5 or 5.0 mg L-1 BA or 2.5 mg L-1 BA + 2.5 mg L-1 of one of NAA, IAA or IBA. Different growth parameters regarding shoot multiplication were recorded. BA at the rate of 2.5 mg L-1 caused the earliest sprouting of buds and produced the longest primary shoot, maximum number of nodes per primary shoot. However, BA at the rate of 5.0 mg L-1 produced the maximum number of shoots per explant. While the combination consisting of 2.5 mg L-1 BA + 2.5 mg L-1 IBA delayed the bud sprouting and produced the minimum number of shoots, the shortest primary shoot and the minimum number of nodes per primary shoot. Among the strains PKJ-3 led the other strains in performance, while PKJ-2 trailed in all parameters. The number of shoots and the length of primary shoot were significantly affected by the interactions between strains and growth regulators combinations.
Muhammad Azhar Bashir, Hamid Rashid and Muhammad Akbar Anjum, 2007. In vitro Shoot Multiplication of Six Promising Strains of Jojoba (Simmondsia chinensis). Biotechnology, 6: 309-315.
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