Twenty one accessions from different diploid species, 10 high yielding varieties each of Triticum durum and Triticum aestivumwere analysed by SDS-PAGE, to study polymorphism among the triticin protein. Also tricin gene fragment, of 21 accessions of different diploid species was amplified by PCR. No polymorphism was found in triticin protein subunits of T. durum and T. aestivum varieties but enough polymorphism was observed in wild wheats. The A-genome species -T. urartu and T. boeticum accessions having molecular weight higher than 1AS coded triticin subunits of Chinese spring can be used for further analysis. Expression of triticin subunits, in S-genome, Ae. longissima and Ae. speltoides and absence of the same in hexaploid wheats (B-gemone coded subunits) suggested that later can be transferred to cultivated wheats for increasing triticin/lysine content.