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Biotechnology
  Year: 2009 | Volume: 8 | Issue: 3 | Page No.: 316-322
DOI: 10.3923/biotech.2009.316.322
Molecular Characterization of Pomegranate (Punica granatum L.) Landraces Grown in Jordan using Amplified Fragment Length Polymorphism Markers
H. Awamleh, D. Hassawi, H. Migdadi and M. Brake

Abstract:
Amplified Fragment Length Polymorphism (AFLP) technique was used to asses the genetic diversity among twelve pomegranate landraces collected from three locations in Jordan. Eight AFLP primer combinations detected a total of 1433 bands with an average of 14.9 bands per landrace. MseI+CTG and EcoRI+ATG primer combinations have the highest ability to discrimination the landraces; they revealed 265 bands with an average of 22.1 band/landrace. The polymorphism that detected by individual primer combinations ranged from 56.7 to 100%. The average genetic similarity ranged from 0.46 to 0.87 among the twelve tested landraces. The highest similarity was recorded between the landraces Qrati and Khdari1, Táeafi and Helow Khashabi. Landrace Zeglabi showed broad diversity comparing to the other landraces. However, all landraces were discriminated with the tested primer combinations. This study has emphasized the ability of AFLP in determining the genetic diversity among pomegranate landraces.
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How to cite this article:

H. Awamleh, D. Hassawi, H. Migdadi and M. Brake, 2009. Molecular Characterization of Pomegranate (Punica granatum L.) Landraces Grown in Jordan using Amplified Fragment Length Polymorphism Markers. Biotechnology, 8: 316-322.

DOI: 10.3923/biotech.2009.316.322

URL: http://scialert.net/abstract/?doi=biotech.2009.316.322

 
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