Abstract:
A strain Bacillus altitudinis ZJ 186 capable of producing antifungal
compounds to inhibit the growth of seven tested physiological races of Magnaporthe
oryzae was isolated from marine soil samples and characterized by biochemical
and physiological identification as well as 16S rDNA sequence analysis. The
filtrate from the culture broth of strain ZJ 186 showed strong growth inhibition
against all tested physiological races of Magnaporthe oryzae indicating
that suppression of the growth of the fungi was due to the presence of antifungal
compounds in the ferment broth. Results showed that the filtrate exhibited antifungal
activity at 20 h after inoculation and became significant correlated with the
cell growth over 96 h cell growth period (p<0.01). Antifungal activity was
relatively stable when the filtrate was exposed to conditions in the pH range
1-8, but was sensitive to alkalinity conditions. Moreover, the antifungal components
lost partial activity when the temperature increased, but kept a quite thermally
stable with approximately 40% of the activity of the culture filtrate being
held at 60, 80 and 100°C for 30 min, respectively. It’s the first report
of bacterium Bacillus altitudinis showing antifungal capability against
Magnaporthe oryzae.
Xi-Biao Jin, Rui-Jie Sun, Jian-Qing Zhu, Zheng-Jun Xu, Zhu Liu, Qiang Wang and Xiao-Ying Ye, 2012. Isolation and Identification of Bacillus altitudinis ZJ 186 from Marine Soil Samples and its Antifungal Activity Against Magnaporthe oryzae. Current Research in Bacteriology, 5: 13-23.
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