Desmids are freshwater algae often considered as indicator of oligotrophic environment for water bodies. There are ample examples of works done by various workers throughout the world. Though desmids are reported from many parts of India, North East India, located in South of the Eastern Himalaya, is lacking behind in the study of this particular microflora in spite of its rich biodiversity. Therefore, an attempt has been made to study the planktonic desmid flora of North East India. Samples are collected with the help of planktonic net, wide mouth bottles and natural periphytons. Species are identified with the help of standard literature. In the present investigation, a total no. of 38 taxa of desmids including 8 species of genera Closterium, 10 species of Cosmarium, 5 species of Euastrum, 5 species of Micrasterias, 1 species of Netrium, Tortitaenia and Gonatozygon, 2 species of Pleurotaenium and 5 species of Staurastrum were recorded as phytoplankton during August 2009 to 2010 which are new records from the South of the Eastern Himalayas. Among them Closterium and Cosmarium are found to be more abundant indicating their oligotrophic nature which are need to be conserved.