The present study aims to study the possible effect
of the cyanobacterium Spirulina sp. as a protective agent in rats
given diethylnitrosamine (DEN) in drinking water. The investigation is
supposed to carried out through histopathological and immunohistochemical
examinations for proliferation marker Ki67 in liver tissues. The hepatoprotective
role of Spirulina shown by the histopathological examination of
the liver where only little dilatation in the blood sinusoids, slight
vacuolization in the cytoplasm and few pyknotic nuclei were observed.
Immunohistochemical evaluation shown reduction of the number of malignant
cells and its proliferation in treated group, but the Ki67 expression
was high labeling index in DEN induced heptocarcinoma group. Also, liver
Ki67 labeling index (Ki67 LI) in tumor tissues or adjacent non-tumor tissues
were higher than that in normal liver tissues, while in tumor tissues
it was higher than that in adjacent non-tumor tissues. So, using Spirulina
seems to have realistic results and rapid curative effect. The development
of specific therapeutic strategies based on natural algal products should
be considered as an attractive approach. Based on these fascinating possibilities,
Spirulina offers a great promise for patients with liver injury
and Ki67 expression could represent a valuable tool in the understanding
of hepatocellular carcinoma.
Mashael Mohammed Bin-Meferij , 2009. Histological and Immunohistochemical Studies for Evaluation of the Role of Microalgae Spirulina sp. Against Cancer in Experimental Animals. International Journal of Cancer Research, 5: 36-43.