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International Journal of Pharmacology
  Year: 2006 | Volume: 2 | Issue: 4 | Page No.: 374-381
DOI: 10.3923/ijp.2006.374.381
Application of Human Lymphocytes for Evaluating Toxicity of Anti-Cancer Drugs
Suman G. and Kaiser Jamil

Abstract:
Important discoveries came forth when human cell lines were used for screening drugs and other chemicals. This investigation determines the toxicity profiles of neoplastic drugs utilizing human lymphocytes from peripheral blood samples of healthy non-smoking donors and the blood samples of cancer patients suffering from acute myeloid leukemia (AML) were collected. The order of toxicities (LC50) using MTT assay was found to be as follows; Cisplatin > Carboplatin> Cyclophosphamide > 5-FU > Vincristine. The values were calculated using statistical analysis. The MTT assay is now widely adopted by researchers and industry, as it is a rapid Spectrophotometric method for determining cell viability in cells. The changes induced by individual drugs could be unique to each compound which might reflect in genotoxicity, hence utilizing the Single Cell Gel Electrophoresis (SCGE) assay, genotoxicity of the drugs was determined as comet tails of DNA damages. It was found that the values obtained were significant (p<0.04) and showed that the damage was highest in cisplatin as compared to other drugs. The order of toxicity in comet assay was similar to that of MTT assay. It is therefore concluded that the two assays could be used simultaneously to determine the toxic properties of the drugs on human cells and can be compared to cancer cells.
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How to cite this article:

Suman G. and Kaiser Jamil , 2006. Application of Human Lymphocytes for Evaluating Toxicity of Anti-Cancer Drugs. International Journal of Pharmacology, 2: 374-381.

DOI: 10.3923/ijp.2006.374.381

URL: http://scialert.net/abstract/?doi=ijp.2006.374.381

 
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