In the present study we have assessed the protective effect of hesperidin a flavonoid against nicotine induced chronic lung damage. The effect on nicotine is assessed by the elevation of marker enzymes, antioxidants and lipid peroxidation in nicotine treated rats. Lung damage was induced by subcutaneous injection of nicotine at a dose of 2.5 mg kg-1 body weight. Hesperidin was administered orally at different doses (25, 50, 75, 100 and 150 mg kg-1 body weight). The level of marker enzymes in plasma and antioxidants in lung, kidney and liver were estimated. Supplementation of hesperidin decreased the levels of all the marker enzymes; it was significant in 25 mg kg-1. The result shows that hesperidin is protective against the lung damage caused by nicotine at 25 mg kg-1.