A natural compound, zerumbone was extracted, isolated
and purified from the rhizomes of edible plant Zingiber zerumbet
using methanol extraction and Column Chromatography (CC) method. The isolated
and purified zerumbone crystals were subjected to High Performance Liquid
Chromatography (HPLC), Liquid Chromatography Mass Spectrometry (LCMS)
and 13C NMR and 1H NMR analysis to confirm the purity, molecular weight
and molecular structure. The study investigated the purified zerumbone
crystals for its anti-cancer properties on human cervical cancer cell
line (HeLa). Cisplatin, was used as a positive control in this study.
The cytotoxicity of zerumbone and cisplatin were investigated using the
MTT assay and caspases-3 was estimated with colorimetric assay in zerumbone
treated HeLa cells. Morphological analysis showed that there were changes
observed on HeLa cancer cells after treatment with zerumbone and cisplatin.
The MTT assay results demonstrated that the IC50 value ( ±
SEM) of zerumbone was determined to be 11.3 μM (2.5 μg mL-1)
whilst the IC50 value of cisplatin was at 7.5 μM (1.6
μg mL-1). Prominent growth retardation was identified
to the HeLa cancer cells, after treatment with both compounds, while caspase-3
was observed to be significantly increased in zerumbone treated cells
as compared to untreated control cells. This study showed promising avenues
towards zerumbone to be developed as a new chemo-natural drug for treatment
of cervical cancer.
A.B.H. Abdul, A.S. Al-Zubairi, N.D. Tailan, S.I.A. Wahab, Z.N.M. Zain, S. Ruslay and M.M. Syam, 2008. Anticancer Activity of Natural Compound (Zerumbone) Extracted from Zingiber zerumbet in Human HeLa Cervical Cancer Cells. International Journal of Pharmacology, 4: 160-168.