Cortisol is involved in a number of physiological and oxidative
processes, including functional regulation in human milk. This hormone has been
widely used for several therapeutic approaches. The aim of this study was to
evaluate the immunomodulatory effect of Polyethylene Glycol (PEG) microspheres
with adsorbed cortisol on the functional activity of colostrum phagocytes. The
PEG microspheres were evaluated by flow cytometry and fluorescence microscopy
were determined in colostrum phagocytes the effects of the PEG microspheres
with adsorbed cortisol on the viability, superoxide release, phagocytosis, microbicidal
activity and intracellular calcium release. The fluorescence microscopy and
flow cytometry analyses revealed that the cortisol was able to adsorb PEG microspheres.
The phagocytes display an increased release of superoxide in the presence of
PEG microspheres with adsorbed cortisol. PEG microsphere adsorbed cortisol increased
the phagocytosis and mediated bacterial killing by colostral mononuclear phagocytes.
The cortisol adsorbed to PEG microspheres had direct effect on colostrum phagocytes
and that they were able to increase the intracellular calcium release of colostrum
phagocytes. These findings suggest that PEG microspheres with adsorbed cortisol
present immunostimulatory effects in colostrum phagocytes and thus, these PEG
microspheres have potential for future clinical applications in the infection
Danny Laura Gomes Fagundes, Eduardo Luzia Franca, Cristiane de Castro Pernet Hara and Adenilda Cristina Honorio-Franca, 2012. Immunomodulatory Effects of Poly (Ethylene Glycol) Microspheres Adsorbed
with Cortisol on Activity of Colostrum Phagocytes. International Journal of Pharmacology, 8: 510-518.