HIV/AIDS has assumed an alarming rate since it was identified and reported in Nigeria. About 3.5 million Nigerians were reported to be living with HIV/AIDS in 2001. This increased to 3.6 million in 2003 and 6 million in 2005. However, the 2008 HIV National Sentinel Seroprevalence Survey among antenatal clinic attendees in Nigeria revealed that 2.9 million people are living with HIV/AIDS in Nigeria, with the least prevalence in Ekiti (1.0%), the highest prevalence in Benue (10.6%) and Kwara (the studied area) with 1.8%. This study aims at assessing a more comprehensive seroprevalence of HIV/AIDS among the general population in Kwara state, Nigeria. This is a retrospective study. Health records on HIV/AIDS of the Kwara State Ministry of Health, HIV/AIDS Control Units were used. The health records comprise of reports from the 16 health facilities providing HIV Counseling and Testing (HCT) Services in the 16 Local Government Areas of the state. The prevalence of HIV/AIDS within the study period among the general population was 5.39%, with near similar prevalence in both sexes (male was 5.47% and female was 5.37%). Results from this study also revealed that more female used the HCT Services in the health facilities. The study shows a higher HIV prevalence among the general population (than the antenatal clinic attendees), but a decline in HIV prevalence in the study population between March, 2007 and December, 2008. HIV prevalence was not different in both sexes, suggesting that both sexes carry equal burden of the disease.