The genetics of plants regenerated from anther-derived callus was investigated using
single crosses & inbreds of maize. Anther culture in maize (Zea mays L.) usually
follows an indirect procedure involving callus induction and differentiation on at
least two different media. Alternatively, development of a direct procedure by which
plantlets can be regenerated directly on one medium seems desirable, if acceptable
frequency of plant regeneration could be obtained and an effective procedure of
chromosome doubling of regenerated plantlets is available. Yupie (YP) and N6 were
used as basal media and 10 growth regulators (2,4-D, kinetin, NAA, IAA, IBA,
dicamba, pCPA, 6-BA, 2ip and hypoxanthine) were tested to evaluate the feasibility
of direct regeneration of haploid plantlets of eight single cross hybrids and four
inbred lines. The results indicated that the direct generation of plantlets is feasible
through anther culture, though it is genotype and medium dependent. Ten plantlets
were regenerated directly from single cross hybrid M60 X M96. The more effective
medium was N6 basal medium plus 2,4-D (2.0 mg l-1) and kinetin (1.5 mg l-1).
Regeneration frequency (percent of anthers producing plantlets) ranged from 1 to 4%.