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Journal of Biological Sciences
  Year: 2002 | Volume: 2 | Issue: 9 | Page No.: 591-595
DOI: 10.3923/jbs.2002.591.595
A Single Step Purification of Gastricsin-Like Proteinase from Atlantic Cod (Gadus morhua)
M. A. Amiza and R.K. Owusu Apenten

Abstract:
A gastricsin-like proteinase was purified from gastric mucosa of Atlantic cod by a single step purification scheme on ion-exchange of Amberlite CG-50. The purification was very efficient as the recovery was 205% and the purification factor was 1796-fold. The enzyme preparation was homogeneous as observed by SDS-PAGE and isoelectric focusing. The enzyme has an estimated molecular weight of 34 kDa and the pI of 4.4.
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How to cite this article:

M. A. Amiza and R.K. Owusu Apenten , 2002. A Single Step Purification of Gastricsin-Like Proteinase from Atlantic Cod (Gadus morhua) . Journal of Biological Sciences, 2: 591-595.

DOI: 10.3923/jbs.2002.591.595

URL: http://scialert.net/abstract/?doi=jbs.2002.591.595

 
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