Callus cultures were initiated from nodal segments on Murashige and Skoog medium supplemented with 2,4-D, BAP and Kn. The highest frequency (85%) of organogenic callus induction was observed in MS medium containing 1 mg L ha-1 BAP and 2 mg L ha-1 Kn. Development of adventitious shoots occurred when the calli were subcultured in MS medium supplemented in the BAP and Kn. Shoots differentiated best (80%) from node derived callus on MS medium containing 1 mg L ha-1 BAP and 2.5 mg L ha-1 Kn. Regenerated shoots rooted best on MS medium containing IBA and Kn (1 mg L ha-1 ). Plantlets were transferred to pots containing sand and soil mixture, acclimatized in a culture room and finally rooted plants were transferred to soil.
N.A. Siddique , M.A. Bari , Sharmin Shahnewaz , M.H. Rahman , M.R. Hasan , M.S.I. Khan and M.S. Islam , 2004. Plant Regeneration of Withania somnifera (L.) Dunal
(Ashwagandha) from Nodal Segments Derived Callus an Endangered Medicinal Plant
in Bangladesh . Journal of Biological Sciences, 4: 219-223.