Antimicrobial Activity of Polysaccharide Isolated from the Cuttlebone of Sepia aculeata (Orbingy, 1848) and Sepia brevimana (Steenstrup, 1875): An Approach to Selected Antimicrobial Activity for Human Pathogenic Microorganisms
Polysaccharide isolated from the cuttlebone of Sepia
aculeata and Sepia brevimana using 10 mM EDTA were studied
for their antibacterial and antifungal activity against nine bacterial
(Bacillus subtilis, Escherichia coli, Klebsiella pneumoniae,
Vibrio cholerae, Vibrio parahaemolyticus, Staphylococcus
aureus, Pseudomonas aeruginosa, Salmonella typhii and
Shigella sp.) and four fungal (Candida sp., Rhizopus
sp., Aspergillus flavusand Aspergillus fumigatus) pathogens
at different concentrations such as 25, 50, 75 and 100% against control.
The activities were found to be increasing with the increasing concentration
of the extracts. No antibacterial activity was recorded against V.
cholerae in all concentrations of S. brevimana. In S. aculeata,
maximum (8 mm inhibition zone in 100%) and minimum (2 mm inhibition zone
in 25%) activity was recorded against E. coli, but in S. brevimana
the highest and lowest activity was recorded as 17 mm (100%) and 2 mm
(25%) (inhibition zone) against P. aeruginosa and E. coli,
respectively. In the antifungal activity study, the highest and lowest
inhibition zones of 12 mm (100%) and 3 mm (25%) were noted against A.
flavus, Candida sp. and A. fumigatus and Rhizopus
sp. respectively, but in S. brevimana, maximum and minimum
activity of 9 mm (100%) and 2 mm (25%) (inhibition zone) were observed
against A. flavus and A fumigatus, respectively. In both
species the cuttlebone polysaccharide showed no activity against Candida
A. Shanmugam, T.S. Mahalakshmi and A. Barwin Vino, 2008. Antimicrobial Activity of Polysaccharide Isolated from the Cuttlebone of Sepia aculeata (Orbingy, 1848) and Sepia brevimana (Steenstrup, 1875): An Approach to Selected Antimicrobial Activity for Human Pathogenic Microorganisms. Journal of Fisheries and Aquatic Science, 3: 268-274.