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Research Journal of Microbiology

Year: 2008 | Volume: 3 | Issue: 5 | Page No.: 308-318
Research Article

Improving Feeding Strategies for Maximizing Polyhdroxybutyrate Yield by Bacillus megaterium

W. Sabra
Division of Microbiology, Department of Botany, Faculty of Science, Alexandria University, Alexandria, Egypt
D.M. Abou-Zeid
Division of Microbiology, Department of Botany, Faculty of Science, Alexandria University, Alexandria, Egypt
The prokaryotic endogenous storage material Poly-β-Hydroxybutyrate (PHB) can be induced to accumulate in bacteria under conditions of unbalanced growth that also stimulate sporulation in endospore forming bacteria. The present study shows that ammonium concentration higher than 0.4 g L-1 inhibits growth and may be responsible for the stationary phase onset of Bacillus megaterium. Hence, in order to expand the growth rate controlled exponential phase (by delaying stationary phase), ammonium limited fed batch cultures were performed at different feeding rates. Under such conditions, a 2.1 fold increase in the specific PHB productivity was recorded (0.19 gPHB/gbiomass*h) compared to batch cultivations (0.09 g/g*h). Although the lowest ammonium feeding rate was accompanied with the lowest growth rate, it resulted in the highest PHB yield. Present study demonstrates that the PHB content of the cells growing under optimized fed batch conditions reached 65% of the cell dry weight, a value that has not been recorded before for bacilli using a synthetic medium.
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W. Sabra and D.M. Abou-Zeid, 2008. Improving Feeding Strategies for Maximizing Polyhdroxybutyrate Yield by Bacillus megaterium. Research Journal of Microbiology, 3: 308-318.

URL: https://scialert.net/abstract/?doi=jm.2008.308.318

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