Proteases represents one of the major groups of industrial enzymes and a number of detergent stable proteases have been isolated and characterized because of its widespread use in detergents. It is worthwhile to screen microbes from new habitats for proteases with novel properties to meet the needs of rapidly growing detergent industry. High-alkaline serine proteases have been successfully applied as protein degrading components of detergent formulations and are subject to extensive protein engineering efforts to improve their stability and performance. Protein engineering has been extremely used to study the structure-function relationship in proteases and led to deeper understanding of the factors influencing the cleaning performance of detergent proteases. This study, discusses the types and sources of proteases with an overview on applications of proteases as laundry detergent additives and some advances in improving the stability and performance of detergent enzymes.