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Research Journal of Microbiology
  Year: 2010 | Volume: 5 | Issue: 3 | Page No.: 199-211
DOI: 10.3923/jm.2010.199.211
Cellulase Production by Aspergillus fumigatus Grown on Mixed Substrate of Rice Straw and Wheat Bran
A.A. Sherief, A.B. El-Tanash and N. Atia

Abstract:
This study aimed to the production of some active cellulases from some local fungi. Twelve Aspergillus species were isolated from some local soil samples. On the basis of cellulolytic activity, Aspergillus fumigatus was selected and used for production of exoglucanase (EXG; EC: 3.2.1.91), endoglucanase (EG; EC: 3.2.1.4), CMCase, β-glucosidase (BG; EC: 3.2.1.21) and xylanase (Xy; EC: 3.2.1.8) by adopting SSF condition using mixed substrate of rice straw amended with wheat bran. Effect of Culture conditions including; incubation period, initial pH, incubation temperature, moisture level, different nitrogen sources, different lignocelluloses as carbon source and different ratios of mixed rice straw and wheat bran (9:1-1:9) were evaluated. The fungus expressed high enzyme production after 4.0 days incubation at moisture level 75%, initial pH 5-6, at 40°C in presence of NaNO3 as an inorganic nitrogen source. The recorded activities were 14.71, 8.51, 0.93, 0.68 and 42.7 IU g-1 for CMCase, β-glucosidase, exoglucanase, endoglucanase and xylanase, respectively.
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How to cite this article:

A.A. Sherief, A.B. El-Tanash and N. Atia, 2010. Cellulase Production by Aspergillus fumigatus Grown on Mixed Substrate of Rice Straw and Wheat Bran. Research Journal of Microbiology, 5: 199-211.

DOI: 10.3923/jm.2010.199.211

URL: http://scialert.net/abstract/?doi=jm.2010.199.211

 
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