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Research Journal of Microbiology

Year: 2017 | Volume: 12 | Issue: 1 | Page No.: 82-89
Research Article

Natural Nodulation and AMF Colonization of Retama raetam and their Impact on Soil Microbial Properties in Arid Regions of Tunisia

Mosbah Mahdhi
Center for Environmental Research and Studies, Jazan University, P.O. Box 114, Jazan, Kingdom of Saudi Arabia
LiveDNA: 216.13300
Neji Mahmoudi
Research Unit, Biodiversity and Valorization of Arid Areas Bioressources (BVBAA), Faculty of Sciences, Gabès University, 6072, Tunisia
Raya Abddaiem
Research Unit, Biodiversity and Valorization of Arid Areas Bioressources (BVBAA), Faculty of Sciences, Gabès University, 6072, Tunisia
Abdelhakim Msaadek
Research Unit, Biodiversity and Valorization of Arid Areas Bioressources (BVBAA), Faculty of Sciences, Gabès University, 6072, Tunisia
Mokhtar Rejili
Research Unit, Biodiversity and Valorization of Arid Areas Bioressources (BVBAA), Faculty of Sciences, Gabès University, 6072, Tunisia
Mohamed Mars
Research Unit, Biodiversity and Valorization of Arid Areas Bioressources (BVBAA), Faculty of Sciences, Gabès University, 6072, Tunisia
Background and Objective: Retama raetam is a wild shrub that plays an important role in the restoration and the maintenance of soil fertility. To our knowledge, this legume has not been examined for its root Arbuscular Mycorrhizal Fungi (AMF) colonization. The aims of this study were to evaluate the natural nodulation and AMF colonization of Retama raetam growing in five soils from arid regions of Tunisia and their Impact on soil microbial properties. Materials and Methods: Legume nodulating rhizobia associated with R. raetam was estimated using the Most Probable Number (MPN) method. The plant mycorrhizal status was assessed by microscopic observation of root fragments after trypan blue staining. The spore density was quantified and was expressed as the total number of spores per 100 g of soil. The effect of soil microorganisms (rhizobia and mycorrhizal fungi) on soil properties was also investigated. Results: Retama raetam was colonized by both Arbuscular Mycorhizal Fungi (AMF) and rhizobia in all studied sites. The mycorrhizal status and nodulation intensity varied between sites. The highest mycorrhizal and nodulation intensity were observed in Bou Hedma National Park. The AMF colonization was positively correlated with soil microbial properties arbuscular mycorrhizal fungi could effectively participate in the rehabilitation of arid ecosystems of Tunisia. Conclusion: Arbuscular mycorrhizal fungi could effectively participate in the rehabilitation of arid ecosystems of Tunisia. Retama raetam could be an important alternative to increase soil fertility and to prevent erosion in degrade lands of Tunisia.
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Mosbah Mahdhi, Neji Mahmoudi, Raya Abddaiem, Abdelhakim Msaadek, Mokhtar Rejili and Mohamed Mars, 2017. Natural Nodulation and AMF Colonization of Retama raetam and their Impact on Soil Microbial Properties in Arid Regions of Tunisia. Research Journal of Microbiology, 12: 82-89.

URL: https://scialert.net/abstract/?doi=jm.2017.82.89

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