The aim of the present study was to investigate the ameliorating effect of vitamin C on atrazine induced testicular toxicity in Capra hircus in vitro. Small pieces (approximately 1 mm3) of testicular tissue were divided into three groups (One control and two experimental groups). One experimental group was supplemented with 100 nmol mL-1 atrazine concentrations and another experimental group was treated with 100 nmol mL-1 atrazine and simultaneously supplemented with 1000 μmol L-1 concentration of vitamin C (Ascorbic acid). Controls were run simultaneously along with all the experimental groups. Harvesting of tissue was carried out after 1, 4 and 8 h of exposure. In the experimental group treated with atrazine at dose level 100 nmol mL-1, several alterations were observed in the seminiferous tubule. After 1 h of exposure duration there was degeneration in germ cells and somatic cells. Pycnotic nuclei which stained darkly with the eosin were clearly observed after 1 h of exposure duration. The numbers of atretic spermatogonia were increased from 24% in control group to 60% after 1 h, from 30 to 66% after 4 h and from 36 to 76% after 8 h of exposure duration. Similar atretogenic changes were also observed in the testicular slices cultured in atrazine+ vitamin C but were milder as compared to atrazine treatment exclusively. Reduction in atretic spermatogonia was recorded from 60 to 32% after 1 h, from 66 to 42% after 4 h and from 76 to 50% after 8 h of supplementation of vitamin C.