The aim of this study was the evaluation susceptibility of multidrug resistance microorganism to Nanosilver by comparing it with Deconex 5% disinfectant substances. Nanosilver is nanoparticle of silver that is recently being used as antibacterial substance in medicine. This is a cross-sectional study that involved 210 multi drugs resistance microorganism and two type of standard references bacterial as Escherichia coli (ATCC-35218 and S. aurous (ATCC 25923) assayed for Nanosilver and Deconex 5% antibacterial susceptibility. Antibiotic susceptible test was done in laboratory of Baqiyatallah Hospital in capital city of Iran. Nanosilver colloid solution brand commercial name 2000sl nanosilver manufactured in Pars Nonacid Company and Deconex 5% (Sterillium) manufacture in Bode-Germany was used. Here 100 g negative and 110 g positive microorganism samples were evaluated. The most common microorganisms were staphylococci, acinetobacter and the most common site of infection was bronchial from ICU admission patients. Staphylococci aurous (ATCC 25923), Escherichia coli (ATCC-35218) and some hospital microorganism were evaluated to susceptibility of Nanosilver, Deconex 5% and antibacterial drugs. This study showed Nanosilver and Deconex can eradicate more than 96 and 100% of multi drug resistance microorganisms, respectively therefore, more evaluation was needed about consumption of Nanosilver for hand washing and hospital environment disinfectant.