The aim of the present study was to investigate the possible public health significance of calves giardiasis in the village. Faecal examination of children and their contact calves revealed infection by Giardia cysts up to 25 and 30.8%, respectively. Protein fraction at molecular weight of 33 kDa as a giardins fraction was found to be closely related to zoonotic strain of calves Giardia using western blot analysis. Amplification and sequencing of a 292 bp fragment of 16S-rRNA ribosomal unit from 25 calves Giardia isolates using nested PCR, revealed that 20% of these isolates were belonging to the zoonotic Giardia Assemblage A, while the rest of the isolates (80%) were in Assemblage of livestock genotype (E). This level of calve zoonotic Giardia genotype demonstrates the possible role of calves as a reservoir for human infection in this village.