The current study was undertaken with the objective that tissue culture conditions need to be optimized for obtaining vigorous shoot growth coupled with modifications in the nutrient medium so as to reduce the cost of nutrient medium. Axillary shoot tips cultured on MS +IBA (2 mg L-1) + KN (3 mg L-1) + sucrose 3% (w/v) + agar-agar 0.8% (w/v) was the best medium for multiple shoot formation with 86.3% shoot apices forming multiple shoots. The sucrose was replaced with table sugar and agar-agar was omitted completely. Out of 6 low-cost media combinations tested, MS liquid medium supplemented with Indole-3-Butyric Acid (IBA) (2 mg L-1) + kinetin (KN) (3 mg L-1) + table sugar 3% (w/v) was found to be the best with 27 shoots/explant. Seventy percent shoots formed roots on half strength MS salts supplemented with IBA (3 mg L-1) + table sugar 3% (w/v) + agar-agar with an average of 5.6 roots per shoot. The study has resulted in the identification of a low-cost medium combination for rapid multiplication of P. kuura with a potential that the technology can be up-scaled to a large-scale production.
H. Sood and R.S. Chauhan, 2009. Development of a Low Cost Micropropagation Technology for an Endangered Medicinal Herb (Picrorhiza kurroa) of North-Western Himalayas. Journal of Plant Sciences, 4: 21-31.
20 February, 2010
Mafatlal M Kher: Nice paper
Its interesting that IBA and KN in combination gives multiple shoot induction Generally in other plant Only Cytokinin or Combinations of Cytokinin is preferable for multiple shoot induction Some times Cytokinin in combinations with Auxin But ratio could be around Auxin approximately .1-1mg per liter and Combination of Cytokinin i.e.Cyto..Concentration around in range of .5-10 mg per liters. it may varies among different plant species