Abstract:
A micropropagation protocol for the important medicinal plant species Dregea volubilis was developed using nodal explants by culturing on Murashige-Skoog (MS) media fortified with different concentration of KIN and BAP. The highest number of multiple shoots (9.36±0.67e) was noticed at the KIN level of 3.0 mg L-1 followed by 8.26±0.62e shoots in KIN 4.0 mg L-1. The in vitro raised micro shoots produced highest percentage of rooting (78%) in MS media augmented with 1.5 mg L-1 IBA. The hardened plantlets were acclimatized in the green house conditions and then reintroduced in the Herbal garden attached to the Centre in which 75% of the plants survived.
N. Yogananth, R. Bhakyaraj, A. Chanthuru and S. Palanivel, 2011. In vitro Regeneration and Conservation of Rare Medicinal Plant Dregea volubilis Benth.. Journal of Plant Sciences, 6: 225-228.