During the present investigation ameliorating effect of vitamin E on endosulphan induced testicular toxicity has been analyzed in Capra hircus in vitro. Vitamin E exhibited the protective role against the damage induced by endosulphan in the testicular tissue. Small pieces (approximately 1 mm3) of testicular tissue were divided into three groups (One control and two experimental groups). One experimental group was treated with 100 nmol mL-1 endosulphan concentration and another experimental group was treated with 100 nmol mL-1 endosulphan and supplemented with 100 μmol L-1 concentration of vitamin E (α-Tocopherol). Harvesting of the testicular tissue was carried out after 1, 4 and 8 h of exposure durations in vitro. Hyalinization and fragmentation was observed in the endosulphan treated group. Chromolysis was observed in spermatogonia, Sertoli cells and spermatids. As the exposure duration enhanced from 4 to 8 h there was elevation in number of pycnotic nuclei, fragmented nuclei, chromolysis of germ cells and somatic cells present in the testis. Endosulphan exposure induced the number of atretic spermatogonia from 24% in control group to 68% after 1 h, from 30 to 76% after 4 h and from 36 to 84% after 8 h of exposure duration. In the experimental group treated with endosulphan and supplemented with vitamin E there was decline in number of pycnotic nuclei, fragmented nuclei and chromolysis as compared with the endosulphan exposed group. There was decline in atretic spermatogonia from 68 to 36% at 1 h, from 76 to 44% after 4 h and from 84 to 58% after 8 h of supplementation duration.
A. Fulia, P.K. Chauhan and R.K. Sharma, 2011. Ameliorating Effect of Vitamin E on Testicular Toxicity Induced by Endosulphan in Capra hircus in vitro. Journal of Pharmacology and Toxicology, 6: 133-140.