The study was carried out for the maximum production of cellulases and hemicellulases at different substrate water ratio and ionic concentration. It was observed that maximum activities of above mentioned enzymes in IU/ml/min. were monitored on 30 per cent substrate: water ratio with 0.2 per cent KH2PO4 and 0.01 per cent MgSO4.7H2O. Maximum FPase and β–glucosidase activities were noted at 0.005 per cent CaCl2 whereas CMCase and xylanase showed maximum activities in the presence of 0.01 and 0.1 per cent CaCl2.
Fathia Mubeen , M. Asghar , S.T.A.K Sindhu , Nusrat Yasmeen and M. Shazia , 1999. Production of Cellulases and Hemicellulases from Wheat Bran by Arachniotus sp. and Their Bioassay . Pakistan Journal of Biological Sciences, 2: 553-554.