Abstract:
All 50 blood samples from Chronic Liver Disease (CLD) patients were screened for (Antibodies against hepatitis C virus) AntiHCV by 3rd generation (Enzyme Linked Immuno-Sorbant Assay) ELISA for diagnosis and genotyping of HCV by (Polymerase Chain Reaction) PCR. Samples were screened by slot blot hybridization using P32 ATP labelled genotype specific oligonucleotide probes indicating prevalence of genotype HCV -1b which was further confirmed by southern hybridization and direct sequencing. Positivity by ELISA was 37% while with that of PCR was 42% in hepatitis patients indicating its sensitivity and reliability and can be used to determine the carrier state and hence its spread can be checked through blood screening.
Khalid Pervez , 2000. Diagnosis and Genotyping of Hepatitis C Virus by Polymerase Chain Reaction
in Chronic Liver Disease Patients
. Pakistan Journal of Biological Sciences, 3: 1098-1099.
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