The isolation, frequency and antibiotic susceptibility pattern of
clinical isolates of Staphylococcus aureus at the Obafemi Awolowo
University Teaching Hospitals, Ile-Ife, was studied. Staphylococcal isolates
obtained from the various specimens were collected from the Microbiology
Laboratory of the Obafemi Awolowo University Teaching Hospital Complex, Ile-Ife
and also from anterior nares of apparently healthy medical personnel of the
hospital. Bacterial identification was based on standard methods which include,
colonial morphology, catalase and gram stain reaction. Confirmatory test was by
coagulase and DNAse tests. The standard disk agar diffusion method was done
employing commercially prepared antibiotic discs (Abtek, Habdiscs) of ampicillin
10 μg, chloramphenicol 10 μg, cloxacillin 5 μg, gentamicin 10 μg, penicillin 1
i.u., streptomycin 10 μg and tetracycline 10 μg. A total of 97 isolates were
obtained from 54 male and 32 female patients and 11 healthy medical personnel. A
high proportion of isolates (58.8%) were recovered from the middle-aged group.
About one-third (28.9%) of all the isolates were obtained from wound samples,
which was mostly recovered in patients less than 20 years. The resistance
profile is shown as follows: penicillin (100%), ampicillin (62%), tetracycline
(45.4%), cloxacillin (47.4%), chloramphenicol (24.7%), streptomycin (23.7%),
gentamicin (22.7%) and erythromycin (11.3%). Thirty isolates (30.9%) were
resistant to at least three classes of antibiotics. There was no significant
difference in resistance to penicillin and chloramphenicol when present data
were compared with an earlier report in Ile-Ife. Low rates of resistance to
erythromycin were noted in this study and previous investigations conducted in
Lagos and Jos, Nigeria. This survey clearly indicates that careful surveillance
of multiple resistant S. aureus is important in infection control and
stringent measures against nosocomial infections associated with
antibiotic-resistant S. aureus are needed in this health institution.
Clinicians should enlighten patients on the consequences of indiscriminate use
of penicillin and other anti-microbial agents.
M.A. Bisi-Johnson , D.O. Kolawole and A.O. Shittu , 2005. Epidemiological Analysis of Clinical Isolates of Staphylococcus aureus in Ile-Ife, Nigeria. Pakistan Journal of Biological Sciences, 8: 1016-1020.