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| Effects of Copper and Superoxide Dismutase Content of Seminal Plasma on Buffalo Semen Characteristics |
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M. Eghbali,
S.M. Alavi-Shoushtari
and S. Asri Rezaii
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Abstract:
To investigate the effects of copper and superoxide
dismutase (SOD) content of seminal plasma on buffalo semen characteristics,
54 semen samples collected from buffalo bulls by a bovine artificial vagina
were used. Semen characteristics (motility, viability, morphology, concentration
and volume) were recorded. Seminal plasma was harvested by centrifugation
and kept frozen until analysis. Seminal plasma copper content was determined
by atomic absorption procedure and SOD was measured by using a kit. The
mean total copper value of seminal plasma was recorded as 2.51 ±
0.04 mg kg-1 (Mean ± SEM) and the mean total SOD values
was 39.02 ± 0.81 IU mL-1. To reduce the range of variability,
the data were categorized according to their motility records in 3 groups
of Excellent (Ex, >90% motile, n = 33), Good (Go, 80-89% motile, n
= 15) and Moderate (Mo, <79% motile, n = 6). The mean motility, viability,
copper and SOD values in Ex group was recorded as 92.24 ± 0.51%,
94.00 ± 0.48%, 2.56 ± 0.04 mg kg-1 and 39.52
± 0.57 IU mL-1, respectively. These values were 81.66
± 0.62%, 85.26 ± 0.95%, 2.38 ± 0.11 mg kg-1
and 36.48 ± 1.51 IU mL-1 in Go group and 71.66 ±
1.05%, 77.00 ± 2.94%, 2.55 ± 0.10 mg kg-1 and
50.66 ± 2.51 in Mo group, respectively. The mean copper value in
Ex group was highly (r = 0.600) correlated with SOD and correlated with
sperm motility (r = 0.372) and viability (r = 0.363), while, in Go group
it was highly correlated (r = 0.945) with SOD and sperm viability (r =
0.652) and in Mo group it was correlated (r = 0.874) with semen volume
only. The mean SOD values in Ex group was highly correlated with sperm
motility (r = 0.492) and viability (r = 0.490) and mean copper values,
in Go group, it was highly correlated whit sperm viability (r = 0.659)
and mean copper values and in Mo group it had no significant correlations
with semen parameters. These results suggest that copper and SOD content
of the buffalo seminal plasma have an influence on the sperm motility
and viability which are the most important factors in semen fertility.
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How to cite this article:
M. Eghbali, S.M. Alavi-Shoushtari and S. Asri Rezaii, 2008. Effects of Copper and Superoxide Dismutase Content of Seminal Plasma on Buffalo Semen Characteristics. Pakistan Journal of Biological Sciences, 11: 1964-1968.
DOI: 10.3923/pjbs.2008.1964.1968
URL: http://scialert.net/abstract/?doi=pjbs.2008.1964.1968
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