Lipase production in an indigenous lipolytic Bacillus
sp. was detected in media containing Tributyrin-Tween 80 and Rhodamine
B-Olive oil. The statistical Taguchi model was used to predict the optimum
experimental conditions for bacterial growth and lipase production. Partial
optimization was carried out for selection of salt base, oil, glucose,
NH4Cl and yeast extract concentrations, inoculum density, pH
and agitation. Maximum lipase activity was detected in the cell free supernatants
of cultures grown in a medium containing 10 g L-1 yeast extract,
15 g L-1 NH4Cl, 3 g L-1 K2HPO4,
1 g L-1 KH2PO4, 0.1 g L-1
MgSO4.7H2O, 2 g L-1 glucose, 0.6 mM MgCl2
and 15 ml L-1 olive oil, pH 8.5 at 30 °C for 24 h and low
agitation. The amount of lipase produced in the designed medium was in
agreement with the predicted values by the statistical method. 16S rRNA
cloning and sequencing identified the test organism as Bacillus pumilus.
Kambiz Morabbi Heravi, Fereshteh Eftekhar, Bagher Yakhchali and Fatemeh Tabandeh, 2008. Isolation and Identification of a Lipase Producing Bacillus sp. from Soil. Pakistan Journal of Biological Sciences, 11: 740-745.