The genetic relationship was examined in a population
of Grus antigone sharpii L. using DNA markers from the ISSR technique
for applying towards breeding purposes for conservation of species. Since
their extinction from Thailand, sixteen eastern sarus cranes: Grus
antigone sharpii L. provided from Cambodia were fed and bred to sixty
individuals at Nakhonratchasima Zoo, Northeastern Thailand to re-exist
in Thai natural sites. Their genetic diversity and distance were examined
to test their possibility to adapt to environmental variation. Blood samples
from 27 individuals of Grus antigone sharpii L. were collected
and DNA was extracted. These DNA samples were amplified using the successful
fifteen from twenty four primers inter simple sequences repeat markers.
A dendrogram was constructed and shows distance values of the species
between 12.1 and 53.5. The samples produced 63.96% polymorphic banding
profiles. The genetic diversity (H′) in this population was estimated
using Shannon′s index. The high H′ value of 0.501 reflected
the somewhat wide range of distribution sites, which would adapt to environmental
variations. Genetic evenness is 0.152. This value supports that all the
studied samples have a small equal genetic abundance.
T. Tanee, A. Chaveerach, A. Anuniwat, A. Tanomtong, K. Pinthong, R. Sudmoon and P. Mokkamul, 2009. Molecular Analysis for Genetic Diversity and Distance of Introduced Grus antigone sharpii L. to Thailand. Pakistan Journal of Biological Sciences, 12: 163-167.