The present investigation was carried out for the estimation of sterol in edible fats and oils. Ten samples of vanaspati ghee which were most commonly available in the market were subjected to sterol estimation. The samples included Dalda, Tullo, Habib, Fauji, Kissan, ACP, Latif, Family, Zaiqa and Handi vanaspati. Dalda, Tullo and Habib vanaspati contained relatively less amount of cholesterol (10, 14 and 15 mg/g respectively) While, Fauji, Kissan, ACP and Latif vanaspati had the intermediate values of cholesterol (24, 25, 29 and 30 mg/g respectively). Family, Zaiqa and Handi vanaspati contained maximum amount of cholesterol (33, 37 and 40 mg/g respectively). Dalda vanaspati had the least (10 mg/g) while, Handi vanaspati had the highest (40 mg/g) amount of cholesterol. Among other samples butter and Haleeb Desi ghee contained the high amount of cholesterol (27, 7 mg/g respectively), while milk fat, beef tallow and fish fat were low in cholesterol content (3.5, 1 and 4 mg/g respectively). Bear fat had the immense quantity of cholesterol (80 mg/g). The sterol content of edible oils i.e. Corn (23 mg/g), Soybean (9 mg/g), Rapeseed (5 mg/g) and Coconut (0.8 mg/g) were reported less as compared to the sterol content of mustard oil (64 mg/g).