The aetiology of a disease characterized by severe leaf
curling and leaf distortion symptoms in peppers was studied in Burkina
Faso. Surveys were conducted in fields planted with sweet pepper (Capsicum
annuum) and hot pepper (Capsicum frutescens) to collect leaf
samples and assess disease incidence. Collected samples were analyzed
using biological tests (mechanical inoculation and whitefly transmission),
triple-antibody-sandwich- enzyme linked-immunosorbent assay (TAS-ELISA),
Polymerase Chain Reaction (PCR) and sequence analysis. Mechanical inoculation
tests were negative while transmission by the whitefly Bemisia tabaci
led to infection rates of more than 80%. TAS-ELISA and PCR tests indicated
that symptoms observed were associated with begomovirus infections. Involved
virus isolates showed pairwise nucleotide identity of more than 99% with
Pepper yellow vein mali virus (PepYVMV) and formed with this virus
a distinct clade in phylogenetic analyses. Altogether, these results indicated
that PepYVMV was the causal agent of leaf curling symptoms in peppers.
Fidele Tiendrebeogo, V.S. Edgar Traore, Nicolas Barro, Alfred S. Traore, Gnissa Konate and Oumar Traore, 2008. Characterization of Pepper yellow vein mali virus in Capsicum sp. in Burkina Faso. Plant Pathology Journal, 7: 155-161.