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Plant Pathology Journal
  Year: 2016 | Volume: 15 | Issue: 2 | Page No.: 65-74
DOI: 10.3923/ppj.2016.65.74
Molecular Cloning and Sequencing of Telosma Mosaic Virus (TeMV) Causing Mosaic Disease on Patchouli
Rita Noveriza, Gede Suastika, Sri Hendrastuti Hidayat and Keiko T. Natsuaki

Abstract:
Patchouli belonging to the family Lamiaceae is one of the important aromatic plants that originated from the Philippines. Telosma mosaic virus (TeMV) is one of the dominant types of viruses which attack Indonesian patchouli causing mosaic symptoms on the leaves. Only few research publications were conducted in Indonesia, hence this study was conceptualized in the information on the genome structure of the virus was not enough. The nucleotides sequence encoding the Coat Protein (CP) of TeMV-I (Indonesia isolate) was cloned and determined to complete information about this virus and designed an original primers for sequencing of the genes of NIb and NIa of TeMV-I. A part of genome structure of TeMV was sequenced from patchouli plants showing mosaic symptoms by using universal primer from the genus Potyvirus, then cloned to pGEM-T vector. Sequence analysis was done with program Sequence Scanner and CLC Sequence Viewer. The result showed that newly primer set for NIa and NIb region was design and cloned to TeMV-I. It was determined a sequence of 3805 nucleotides (nt) or a polypeptide chain of 1193 amino acid (aa) at the 3’-terminal region of the genome of TeMV. This contained the 3’-terminal part of the NIa (nts 1-1182), the NIb (nts 1183-2733), the coat protein (nts 2734-3549) and the 3’-UTR (nts 3550-3805). The stop codon (TAA) was followed by a 3’-untranslated region (3’-UTR) of 253 nt. The sequence analysis and development of specific primers for TeMV will be of great help in the detection and protection of this virus.
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How to cite this article:

Rita Noveriza, Gede Suastika, Sri Hendrastuti Hidayat and Keiko T. Natsuaki, 2016. Molecular Cloning and Sequencing of Telosma Mosaic Virus (TeMV) Causing Mosaic Disease on Patchouli. Plant Pathology Journal, 15: 65-74.

DOI: 10.3923/ppj.2016.65.74

URL: http://scialert.net/abstract/?doi=ppj.2016.65.74

 
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