The present investigation deals with antioxidant activities of the ethanolic extract of C. asiatica fresh leaves, P. granatum seeds, G. glabra dry root and A. catechu nuts. The effects of all ethanolic extracts of the herbs were studied via reducing power estimation method. The antioxidant activities of the extracts were compared with standard i.e., of ascorbic acid. The equal amount of extract and ascorbic acid combination antioxidant activity was also studied to know the synergistic effect of chemical and extract in any pharmaceutical and cosmetic formulations. The individual antioxidant activity of C. asiatica, P. granatum, G. glabra and A. catechu were found to be 8.23±0.12, 24.35±0.25, 24.25±0.52, 31.31±1.80%, respectively to the standard indicating antioxidant activity to all ethanolic extracts, but catechu showed highest activity (p<0.05). The combined antioxidant activity (ascorbic acid with extracts) showed additive synergistic effect as compared to standard i.e., 110.51±0.422, 127.51±0.745, 128.09±0.235, 134.73±0.60, respectively with each extract. These studies may suggest that the combination of chemical with extract as antioxidant can be utilized in pharmaceutical and cosmetic formulation or chemical antioxidant replaced by herbal natural antioxidants.
M.S. Ashawat , Saraf Shailendra and Saraf Swarnlata , 2007. In vitro Antioxidant Activity of Ethanolic Extracts of Centella asiatica, Punica granatum, Glycyrrhiza glabra and Areca catechu. Research Journal of Medicinal Plant, 1: 13-16.