Effect of Sample Pre-enrichment and Characters of Food Samples on the Examination for the Salmonella by Plate Count Method and Fluorescent in-situ Hybridization Technique
Abstract:
In most of the novel and traditional methods used in the examination of food samples for Salmonella, pre-enrichment of samples is used as a means of increasing the sensitivity and reliability. However, the influence of pre-enrichment of sample on some of those methods has not been studied. Furthermore, the effect of the conditions of the sample on the sensitivity of some of those methods are also not been studied. The aim of this research was to study the influence of pre-enrichment and the conditions of the samples on the detection sensitivity of one novel method Fluorescent in-situ Hybridization (FISH) and one traditional culture techniques (XLT-4 agar plates). To study the influence of enrichment, 60 pork sausage samples collected from 20 different food outlets were examined for Salmonella contamination by both methods with and without pre-enrichment. To study the effect of the conditions of food, collected samples were spiked with 107 cfu mL-1Salmonella enterica culture and examined with and without pre-enrichment. Detection sensitivity of both methods was higher in pre-enriched fresh samples as well as spiked samples. FISH method was found to be more sensitive and less affected by the conditions of food, compared to culture method.
R.M.U.S.K. Rathnayaka , 2011. Effect of Sample Pre-enrichment and Characters of Food Samples on the Examination for the Salmonella by Plate Count Method and Fluorescent in-situ Hybridization Technique. American Journal of Food Technology, 6: 851-856.
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