In this study, a homemade laser Raman spectrometer was
used to examine the Raman spectra of different edible oils, such as canola,
corn, soybean, sunflower, palm and coconut oils. To avoid severe fluorescence
effects which tend to mask the weak Raman lines, Raman bands in the 2800-3200
cm-1 region resulting from unsaturated and saturated carbon
bonds in the fatty acids were chosen and measured the TUSFA/TFA (total
unsaturated fatty acid/total fatty acid content) ratios of the various
samples. Good correlation of the ratios of TUSFA/TFA of different edible
oils with that calculated from manufacturers data was obtained, enabling
us to measure unsaturation/total fatty acids ratio quickly and easily for any
unknown oils and hence the measure the degree of possible adulteration.
S. Farid Uddin Farhad, K.M. Abedin, M. Rafiqul Islam, Aminul I. Talukder and A.F.M.Y. Haider, 2009. Determination of Ratio of Unsaturated to Total Fatty Acids in Edible Oils by Laser Raman Spectroscopy. Journal of Applied Sciences, 9: 1538-1543.