The importance of the reuse of sewage sludge is derived from its high nutrient content that can improve the characteristics of the soil and kind of crop. Accordingly, it is important to characterize the genotoxicological potential of sewage sludge amended soil with such valuable properties "benefits". Field experiment was conducted to evaluate the residual effect of previous applications of sewage sludge used as biofertilizer on M2 meiocytes of Zea mays with different sludge rates (10, 20, 30 and 40 T/F for one and two additions). The resulting data demonstrated that M2 plants still have mutations in pollen mother cells. The total percentage of aberrant mother cells increased significantly with increasing sewage sludge concentration either by residual one or two additions. Aberrations was restricted to Metaphase one of Division one (DI) in the two groups of treatments, while, aberrant end product was the most frequent stage in Division two (DII). The most frequent kinds of aberrations were abnormal chromosome distribution and orientation, sticky figures, abnormal spindle behavior, overlapping cells, asynchronized cells and abnormal cell contour.