The total phenolic and flavonoid contents, as well as the in vitro radical scavenging potentials of extracts of Trametes lactinea collected from forest near the Teaching and Research farm of the Federal University of Technology, Akure were assessed. The acetone extract possessed higher total phenolic content (3.21 mg GAE g1), whereas, the methanol extract had higher total flavonoid content (1.46 mg RE g1). All the extracts were able to scavenge 1, 1-diphenyl-2-picrylhydrazyl (DPPH), nitric oxide (NO), hydrogen peroxide (H2O2) and hydroxyl (OH) radicals. At a concentration of 2 mg L1 the extracts produced activities greater than 50%. The observed result indicates that this wild macrofungus, Trametes lactinea could be a valuable source of natural antioxidants that can protect against free radicals mediated damages.