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Asian Journal of Animal and Veterinary Advances
Year: 2014  |  Volume: 9  |  Issue: 7  |  Page No.: 428 - 439

In vitro Maturation, Fertilization and Embryo Development of Immature Buffalo Oocytes Vitrified by Different Cryodevice Types

W.A. Khalil, Sh. A. Gabr, Sh. M. Shamiah, A.M.A. El-Haif and A.E. Abdel-Khalek    

Abstract: This study aimed to compare the efficiency of three cryodevices (Conventional Straws (CS), Hemi-Straws (HS) and spatula (SP)) in vitrification of buffalo oocytes. Oocytes were recovered from ovaries of slaughtered buffaloes. They were vitrified and thawed according to each cryodevice used. Then they were in vitro matured, fertilized and cultured to blastocyst stage. Survival and quality, in vitro maturation, fertilization rate and blastocyst production rates of vitrified immature buffalo oocytes were assessed. Results showed that total survival rate of the vitrified oocytes was higher (p<0.05) for SP and HS (96 and 95%) than CS (80%). Recovery rate of normal oocytes relative to vitrified or survival oocytes was the highest (p<0.05) for SP compared to other cryodevices. In vitro maturation rate (oocytes at metaphase II stage) was lower (p<0.05) for all vitrified oocytes than fresh (61.1 vs. 27.8-44%). SP showed higher (p<0.05) maturation rate (44%) than HS and CS (34.4 and 27.8%, respectively). Cleavage rate was lower (p<0.05) for all vitrified oocytes than fresh oocytes (37.7-58.9% vs. 70.8), being higher (p<0.05) for SP and HS (55.8 and 58.9%, respectively) than for CS (37.7%). Blastocyst production rate relative to cleaved oocytes was lower (p<0.05) for vitrified than fresh oocytes (5.9-14.3% vs. 31.3) but the differences among vitrification cryodevice were not significant. In conclusion, immature buffalo oocytes can vitrified successfully by spatula, conventional straws and Hemi-straws. Spatula device is a reliable alternative, inexpensive and easy to assemble for vitrification of immature buffalo oocytes. Also, vitrification spatula has the largest effective holding capacity.

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