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Articles by Ensaf G. Taha
Total Records ( 2 ) for Ensaf G. Taha
  Essam S. Soliman , Ensaf G. Taha , M.A.A. Sobieh and P.G. Reddy
  Combined effects of temperature, relative humidity and litter pH in the presence or absence of organic matter on the survival of S. typhimurium over time was studied. The litter (L: 30 cm x W: 25 cm x D: 6 cm aluminum trays filled with wood shavings) was inoculated with S. typhimurium at initial concentration of 4.8 x 107CFU/ml, then litter trays were placed in a room with microclimate similar to that of a naturally ventilated poultry house. The periodical measurement of S. typhimurium population in poultry litter in relation to the ambient environmental conditions revealed that: in the absence of organic matter; there was a non-significant (p<0.99) negative correlation (-0.07 at confidence level 95%) between ambient temperature and survival of S. typhimurium, a non-significant (p<0.53) positive correlation (+0.04 at confidence level 95%) between relative humidity and survival of S. typhimurium population and a highly significant (p<0.005) positive correlation (+0.67 at confidence level 95%) between litter pH and survival. In the presence of organic matter, there was a non-significant (p<0.55) negative correlation (-0.22 at confidence level 95%) between ambient temperature and survival, a highly significant (p<0.0001) negative correlation (-0.12 at confidence level 95%) between relative humidity and survival and a significant (p<0.05) positive correlation (+0.48 at confidence level 95%) between litter pH and survival. The study suggested that increased litter pH and relative humidity rather than temperature presented a great influence on the increased survival of S. typhimurium. New management practice that will reduce litter pH and relative humidity should be considered in the control plans of Salmonellosis in poultry farms.
  Ensaf G. Taha , A. Mohamed , K.K. Srivastava and P.G. Reddy
  The main objective of this study was to standardize and compare rapid methods for the detection of Salmonella in meat samples using Immuno-Magnetic Separation (IMS) followed by culturing in CHROMagar Plus media, Enzyme-Linked Immunosorbent Assay (ELISA) and Real-Time Polymerase Chain Reaction (RT-PCR). Ten-fold dilutions of bacterial suspension (S. typhymurium, ATCC13311) were prepared from the original concentration of 1.6 x 106cfu/ml. Chicken wing samples of 25 g each negative for Salmonella were spiked with six different concentrations of bacteria ranging from 106 to 101. These samples were incubated in buffered peptone water for 4 h as pre-enrichment step and were tested repeatedly. The IMS technique involved the use of paramagnetic polystyrene microscopic beads coated with purified antibodies against Salmonella. The CHROMagar Plus media containing chromomeric substrate facilitated detection of Salmonella species from other flora. The Assurance EIA Salmonella Kit with polyclonal antibodies directed against Salmonella facilitated easy and rapid detection. In the RT-PCR primers targeting invA gene was used which amplified a 378 bp fragment. Comparing to conventional culture method (4 days), CHROMagar plate culture following IMS showed light mauve to mauve-colored colonies of Salmonella in 23 h with high sensitivity (99%) at 1.6 cfu/ml. IMS-ELISA combination also showed high sensitivity (75%) at 1.6 x 103 cfu/ml in 8 h and minimized cross-reactivity with many Enterobacteraceae. The combination of IMS with RT-PCR took less than 7 h and was even more sensitive (100%) at 1.6 cfu/ml. Sensitivities of IMS-RT-PCR and IMS-CHROMagar were higher compared to IMS-ELISA. IMS-CHROMagar was easier to perform and detects only living Salmonella. These methods will be highly suitable for routine detection and may significantly assist the processing industry in avoiding costly recalls and the timely investigation of outbreaks.
 
 
 
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